The purpose of this study is for determination of dental calculus forming factors, stimulation time and periodontal diseaserelated changes in paraffin stimulated whole salivary components and calcium/phosphorus ratio of supragingival and subgingival calculus from dental calculus formers with periodontal disease.
For this study, total 22 persons were divided into two groups ; experimental group were 10 patients with dental calculus and periodontal disease and control group were 12 dental students with well controlled oral hygiene.
Paraffinstimulated whole saliva was collected under clinical condition for first and second 5 minutes for determination of stimulating timerelated change in two groups.
Paraffinstimulated saliva for total 10 minutes was used for determination of dental calculus forming factors and periodontal diseaserelated changes in two groups.
Supragingival calculus and subgingival calculus were collected from patients with dental calculus and periodontal disease.
Stimulated whole saliva was analyzed for pH, flow rate, protein, inorganic phosphorus, and calcium, and calculus was analyzed for calcium/phosphorus ratio.
The attained results were as follows ;
1. A significant stimulating timerelated increase in stimulated whole salivary pH was found in both experimental and control group (P <0.01).
2. A significant stimulating time and diseaserelated decrease in stimulated whole salivary protein was found between experimental and control group (P <0.05).
3. No significant stimulating time or diseaserelated difference in stimulated whole salivary calcium and inorganic phosphorus were found in/or between experimental and control group.
4. A significant difference in stimulated whole salivary flow rate, protein and pH were found between calculus formers with periodontal disease and noncalculus formers with well control. led oral hygiene (P <0.05) .
5. Quantitative analysis have shown slight difference in the content of calcium between supragingival calculus and subgingival calculus.
The Ca/P ratio of supragingival calculus is 1.82 while that of subgingival calculus is 1.72.
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